Publications, presentations and education


Publications

1. Blossom HE, Andersen NG, Rasmussen SA, Hansen PJ (2014) Stability of the intra- and extracellular toxins of Prymnesium parvum using a microalgal bioassay. Harmful Algae 32: 11-21
2. Moestrup Ø, Hansen G, Daugbjerg N, Lundholm N, Overton J, Vestergård M, Steenfeldt SJ, Calado A Hansen PJ (2014) The dinoflagellates Pfiesteria shumwayae and Luciella masanensis cause fish kills in recirculation fish farms in Denmark. Harmful Algae 32: 33-39.
3. Blossom HE, Andersen NG, Rasmussen SA, Larsen TO, Nielsen KF, Hansen PJ (2014) Prymnesium parvum revisited: relationship between allelopathy, ichthyotoxicity, and chemical profiles in 5 strains. Aquatic Toxicology 157: 159-166
4. Eckford-Soper L, Daugbjerg N (2015) Examination of six commonly used laboratory fixatives in HAB monitoring programs for their use in quantitative PCR based on Taqman probe technology. Harmful Algae 42: 52-59
5. Eckford-Soper L, Daugbjerg N (2015) Development of a multiplex real-time qPCR assay for simultaneous enumeration of up to four marine toxic bloom-forming microalgal species. Harmful Algae 48: 37-43
6. Andersen NG, Hansen PJ, Engell-Sørensen K, Nørremark LH, Andersen P, Lorenzen E, Lorenzen N (2015) Ichthyotoxicity of the microalga Pseudochattonella farcimen under laboratory and field conditions in Danish waters. Diseases of Aquatic Organisms 116: 165-172
7. Andersen NG, Lorenzen E, Lorenzen N, Boutrup TS, Hansen PJ (2016) Sublethal concentrations of the ichthyotoxic alga Prymnesium parvum increases the susceptibility of rainbow trout (Oncorhynchus mykiss) to viral haemorrhagic septicaemia virus (VHSV). Diseases of Aquatic Organisms 117: 187-195
8. Eckford-Soper L, Daugbjerg N (2016). A quantitative real-time PCR assay for the identification and enumeration of the occasionally co-occurring ichthyotoxic Pseudochattonella farcimen and P. verruculosa (Dictyochophyceae) and analysis of variation in gene copy number during the growth phase of single and mixed cultures. Journal of Phycology 52: 174-183.
9. Rasmussen SA, Andersen A, Andersen NG, Nielsen KF, Hansen PJ, Larsen TO (2016) Chemical diversity, origin and analysis of microalgal toxins. Journal of Natural Products 79: 662-673
10. Rasmussen SA, Meier S, Andersen NG, Blossom HE, Duus JØ, Nielsen KF, Hansen PJ, Larsen TO (2016). Chemodiversity of Ladder-Frame Prymnesin Polyethers in Prymnesium parvum. Journal of Natural Products 79: 2250−2256
11. Eckford-Soper LK, Daugbjerg N (2016). The ichthyotoxic genus Pseudochattonella (Dictyochophyceae): distribution, toxicity, enumeration, ecological impact, succession and life history – a review. Harmful Algae
12. Eckford-Soper LK, Daugbjerg N (2016) Interspecific competition study between Pseudochattonella farcimen and P. verruculosa (Dictyochophyceae) - two ichthyotoxic species that co-occur in Scandinavian waters. Microbial Ecology.doi:10.1007/s00248-016-0856-z
13. Andersen AJC, Hansen PJ, Jørgensen K, Nielsen KF (2016) An Unbiased Method for Identifying A+2 Element Containing Compounds in Liquid Chromatographic High-Resolution Mass Spectrometric Data. Analytic Chemistry 88: 12461-12469
14. Rasmussen SA, Nielsen KF, Hansen PJ, Larsen TO (2016) Fiskegiftige algetoksiner. Dansk Kemi 9: 18-21.
15. Rasmussen SA, Binzer SB, Hoeck C, Meier S, Medeiros LS, Andersen NG, Place AD, Nielsen KF, Hansen PJ, Larsen TO (2017) Karmitoxin: An Amine-Containing Polyhydroxy-Polyene Toxin from the Marine Dinoflagellate Karlodinium armiger. Journal of Natural Products


Submitted

 


In prep (working titles)

16. Bergsson H, Andersen NG, Hansen PJ, Steffensen JF (2017). The toxic haptophyte Prymnesium parvum reduces oxygen extraction in European plaice (Pleuronectes platessa) (manuscript)
17. Binzer SB, Lundgren LBC, Berge T, Hansen PJ, Vismann B. (2017) Effects of the dinoflagellate Karlodinium armiger on adult and early life stages of the blue mussel Mytilus edulis. Marine Ecology Progress Series
18. Andersen A.J.C. et al. (2017). LC-HRMS Method for the quantitative analysis of the ichthyotoxic polyketide karmitoxin. Analytical and Bioanalytical Chemistry
19. Andersen A.J.C. et al. (2017) Novel polyketides from Alexandrium tamarense
20. Andersen NG, Svendsen DK, Rasmussen SA, Nielsen KF, Larsen TO, Hansen PJ (2017) Relationship between Ichthyotoxicity and gill cell toxicity of the haptophyte Prymnesium parvum
21. Svendsen MBS, Andersen NG, Hansen PJ, Steffensen JF (2017) Determination of oxygen limitation in fishes: Insights from short term Prymnesium parvum exposure
22. Svendsen MBS, Andersen NG, Hansen PJ, Steffensen JF (2017) Fish Acute Toxicity syndrome: Application to Prymnesium parvum exposure
23. Svendsen MBS, Andersen NG, Hansen PJ, Steffensen JF(2017) Swimming performance /behavior and delayed mortality in rainbow trout exposed to Prymnesium parvum
24. Svendsen MBS et al (2016/17). Itchyotoxic modes of action of Alexandrium monilatum
25. Svendsen MBS, Andersen NG Hansen PJ, Steffensen JF(2017). Before hypoxia comes harmful algae: Physiological impacts on fish by harmful algal blooms of Prymnesium parvum

Presentations (presentations, posters)

2012
Hansen PJ (2012) HABFISH. Projekt-ide og struktur. Dansk Akvakultur-møde. 6. marts
Larsen TO (2012) Integrated analytical approaches towards toxic algal natural products discovery. International conference on Harmful Algae, South Korea, October
Andersen NG et al (2012) Sublethal concentrations of the ichthyotoxic alga Prymnesium parvum increases the susceptibility of rainbow trout (Oncorhynchus mykiss) to viral haemorrhagic septicaemia virus (VHSV) International conference on Harmful Algae, South Korea, October 2012

2013
Hansen PJ (2013) HABFISH. DTU System Biology, March 2nd
Blossom HE (2013) The first direct evidence of a distinction between ichthyotoxins and allelochemicals in five strains of Prymnesium parvum. Øresund Plankton Meeting. November 13th.
Andersen NG (2013) Opblomstringer af den fiskedræbende alge Pseudochattonella farcimen i danske farvande og dens skadelige effekter på opdrættede fisk. Foredrag ved Dansk Havforskermøde 2013, Roskilde Universitetscenter.
Daugbjerg, N. (2013) Fiskedræbende mikroalger er blevet et potentielt problem i Danmark. Foredrag ved Dansk Havforskermøde 2013, Roskilde Universitetscenter.

2014
Hansen PJ (2014) Harmful algae, toxins and fish kills. German Society of Protozoologists. Invited presentation. Februar 12th.
Blossom HE (2014) The use of toxins in phytoplankton. DTU ocean life meeting. May 14th
Moestrup Ø (2014) Harmful algae, a world-wide phenomenon which requires world-wide multidisciplinary cooperation. Invited plenary lecture. International Conference on Harmful algae, New Zealand, October 28th
Andersen AJC, Rasmussen SA, Blossom HE, Andersen NG, Hansen PJ, Larsen TO, Jørgensen K, Nielsen KF (2014) Chemotaxonomic Studies of Alexandrium and Karlodinium Using High Resolution Mass Spectrometry. Poster. International Conference on Harmful algae, New Zealand, October 31st
Blossom HE, Andersen NG, Rasmussen SA, Larsen TO, Nielsen KF, Hansen PJ. Prymnesium parvum revisited: relationship between allelopathy, ichthyotoxicity, and chemical profiles in 5 strains. Poster. International Conference on Harmful algae, New Zealand, October 31st
Hansen PJ (2014) Havet mikroskopiske kødædende planter. Selskabet for Naturlærens Udbredelse. 17. november
Andersen NG (2014) Hvorfor slår giftige alger fisk ihjel? Dansk Selskab for Marinbiologi, 2. december.
Blossom HE (2014) The use of toxins in phytoplankton. Dansk Selskab for Marinbiologi, December 2nd.
Hansen PJ (2014) Giftige alger og fiskedød i Danmark: Udbredelse, giftstoffer og baggrunden for fiskedød. Dansk Selskab for Marinbiologi. 2. december.

2015
Mogensen SB, Hansen PJ, Vismann B, Berge T, Andersen NG (2015) De toksiske egenskaber for Karlodinium spp. Foredrag ved det 18. Danske havforskermøde, 28-30. Januar 2015 i København
Fassel NAM, Hansen PJ (2015) Toxic effects of 12 strains of Alexandrium pseudogonyaulax and Alexandrium tamarense on fish larva and gill cells and possible causative toxins. Poster ved det 18. Danske havforskermøde, 28-30. Januar 2015 i København.
Svendsen MBS, Andersen NG, Hansen PJ, Steffensen JF. Before hypoxia comes Harmful Algae: Physiological impacts on fish by Harmful Algal Blooms. Poster at EU Cost Conference. March 2015
Larsen TO, Rasmussen SA, Nielsen KF, Meier S, Duus JØ, Blossom HE, Andersen NG, Hansen PJ. Discovery of fish killing toxins from the microalga Prymnesium parvum. Oral presentation, European Marine Chemists Conference, Glasgow. August 31-September 4, 2015

2016
Nielsen KF (2016) UHPLC-HRMS based dereplication: from fungi to microalgae. The 17th International Conference on Harmful Algae, Brazil 2016. Oral presentation
Rasmussen SA, Meier S, Blossom HE, Andersen NG, Duus JØ, Nielsen KF, Hansen PJ, Larsen TO. Discovery of novel B-type prymnesins from the fish-killing microalgae Prymnesium parvum. The 17th International Conference on Harmful Algae, Brazil 2016. Oral presentation
Andersen NG, Svendsen DK, Rasmussen SA, Nielsen KF, Larsen TO, Hansen PJ (2016) Relationship between Ichthyotoxicity and gill cell toxicity of the haptophyte Prymnesium parvum. The 17th International Conference on Harmful Algae, Brazil 2016. Oral presentation
Moestrup Ø (2016) The IOC-UNESCO Taxonomical reference list of harmful microalgae, status, and state of the art. The 17th International Conference on Harmful Algae, Brazil 2016. Oral presentation
Rasmussen SA, Binzer SB, Meier S, Medeiros LS de, Andersen NG, Place AR, Nielsen KF, Hansen PJ, Larsen TO (2016) Isolation and structure elucidation of the novel cytotoxic super chain polyether karmitoxin from Karlodinium armiger. The 17th International Conference on Harmful Algae, Brazil 2016. Oral presentation-Ignite talk
Andersen AJC, Hansen PJ, Jørgensen K, Larsen TO, Nielsen KF (2016) Isotopic variances filtering for screening Prymnesium parvum extracts for ichthyotoxic metabolites using high resolution mass spectrometry. The 17th International Conference on Harmful Algae, Brazil 2016. Oral presentation-Ignite talk
Svenssen DK, Nielsen KF (2016) Quantification of ichthyotoxic compounds in water samples. The 17th International Conference on Harmful Algae, Brazil 2016. Poster
Binzer SB, Andersen AJC, Medeiros LS de, Rasmussen SA, Svenssen DK, Nielsen KF, Larsen TO, Hansen PJ (2016) The ichthyotoxicity of Karlodinium armiger. The 17th International Conference on Harmful Algae, Brazil 2016. Poster

2017
Binzer SB, Andersen AJC, Medeiros LS de, Rasmussen SA, Svenssen DK, Nielsen KF, Larsen TO, Hansen PJ (2017) The ichthyotoxicity of Karlodinium armiger. Poster. Det 17 Havforskermøde, Helsingør
Varga E, Rasmussen SA, Diernæs L, Larsen TO, Hansen PJ, Nielsen KF (2017) Investigations on prymnesin type-C toxins produced by Prymnesium parvum. Poster. Det 17. Danske Havforskermøde, Helsingør
Hansen PJ, Andersen P (2017). HABFISH - Research in ichthyotoxic microalgae. Poster. Det 17 Danske Havforskermøde, Helsingør.

Education

PhD course (KU/DTU):
International PhD course: Harmful Algae, toxins and fish kills. June 2014. 18 PhD students participated.
MSc courses (KU):
Marine microbiology and virology (2014-2016). ~16 students working for 1 week on harmful algae and bioassays
Applied Marine Biology (2012-2016). ~50 students, 4 days working on toxic algae and the use of bioassays in the search for ichthyotoxins

PhD graduates:
1. Silas Anselm Rasmussen (2015). Thesis title: Discovery of ichthyotoxic natural products from marine microalgae
2. Morten Bo Søndergaard Svendsen (2016). Thesis title: Effects of harmful algae on the physiology of fishes. PhD defense, March 31, 2017

MSc graduates:
1. Heidrikur Bergsson (2014). Thesis title: Respiration in European placice (Pleuronectes platessa); including a sect ion on how the toxic haptophyte Prymnesium parvum reduces oxygen extraction in European plaice (Pleuronectes platessa).
2. Thomas Bech-Thomassen (2014). Thesis title: Assaying assays: The use of Artemia sp. and rainbow trout gill cell line assays in the search for ichtyotoxins in five strains of Prymnesium parvum.
3. Maria Laugmann Laubacher (2014). Thesis title: Physiology and phylogeny of Prymnesium parvum (Prymnesiophyceae), including detection and fast enumeration using real time qPCR.
4. Ronni Ridderholt (2015). Thesis title: The effects of Prymnesium parvum on mussels
5. Sofie Bjørnholt Binzer (2015). Thesis title: The ichthyotixicty of Karlodinium armiger
6. Nicolai Andreas Munk Fassel (2015). Thesis title: The ichthyotoxicity of Alexandrium tamarense and A. pseudogonyalax

Outreach

Newspaper articles, announcements, etc.
2017:
Danskere vil redde fisk fra giftige alger. Indlæg på Innovationsfondens hjemmeside. Link
Danskere vil redde fisk fra giftige alger. Indlæg i Videnskab.dk. Link
2013:
Alger er skyld i fiskedød. Kristeligt Dagblad. 23. januar. Link
2012:
Orbicon: Dialog
JyllandsPosten. Havbrug. Biologi-Newton 11. 22. januar.
2011:
KU-nyhedsbrev. 13. december. Link